Detection of miR-323a-3p in BCa TMAs

A 5′-DIG and 3′-DIG-labeled, locked nucleic acid-incorporated miRNA probe (miRCURY LNATM Detection probe, Exiqon, Woburn, MA, USA) was applied for the detection of miR-323a-3p in BCa TMAs. The probe sequence of hsa-mir-323a-3p was designed as follows: 5′-AGAGGTCGAC CGTGTAATGTG-3′. The specific manipulations were performed as previously described.^{48 (link)} Both the intensity and proportion of positive cells were considered for the semi-quantification of the strength of positivity.

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Li J., Xu X., Meng S., Liang Z., Wang X., Xu M., Wang S., Li S., Zhu Y., Xie B., Lin Y., Zheng X., Liu B, & Xie L. (2017). MET/SMAD3/SNAIL circuit mediated by miR-323a-3p is involved in regulating epithelial–mesenchymal transition progression in bladder cancer. Cell Death & Disease, 8(8), e3010-.

Publication 2017

miRCURY LNATM Detection probe

Cells Mirna Nucleic acid probe

Corresponding Organization :

Other organizations : Zhejiang University, Zhejiang Provincial People's Hospital, Tongde Hospital of Zhejiang Province

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Variable analysis

independent variables

A 5′-DIG and 3′-DIG-labeled, locked nucleic acid-incorporated miRNA probe (miRCURY LNATM Detection probe, Exiqon, Woburn, MA, USA) was applied for the detection of miR-323a-3p in BCa TMAs.

dependent variables

Both the intensity and proportion of positive cells were considered for the semi-quantification of the strength of positivity.

control variables

The specific manipulations were performed as previously described.

controls

No positive or negative controls were explicitly mentioned.

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