Measuring MMP in T. gondii-infected HFF cells

The measurement of MMP was as described elsewhere (Adeyemi et al. 2017 (link)). Briefly, growing HFF cells were treated with C. gigantea oil in the absence/presence of T. gondii infection. After 24 h incubation at 37 °C and 5% CO₂ atmosphere, the cells were harvested, purified, and stained with 200 nM MitoRed (Dojindo Molecular Technologies Inc. Japan) by following the manufacturer’s protocol. Fluorescence measurement was acquired by using a spectrofluorometer (Corona Electric, Japan) with excitation set at 560 nm and emission at 580 nm.

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Atolani O., Oguntoye H., Areh E.T., Adeyemi O.S, & Kambizi L. (2019). Chemical composition, anti-toxoplasma, cytotoxicity, antioxidant, and anti-inflammatory potentials of Cola gigantea seed oil. Pharmaceutical Biology, 57(1), 154-160.

Publication 2019

MitoRed spectrofluorometer

Atmosphere Cells Electric Fluorescence Infection

Corresponding Organization : University of Ilorin

Other organizations : Landmark University, Cape Peninsula University of Technology

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Variable analysis

independent variables

C. gigantea oil

dependent variables

MMP (Mitochondrial Membrane Potential)

control variables

Incubation time (24 h)
Incubation temperature (37 °C)
Incubation atmosphere (5% CO2)
Cell type (HFF cells)
Staining method (200 nM MitoRed)
Measurement method (spectrofluorometer with excitation at 560 nm and emission at 580 nm)

controls

Absence/presence of T. gondii infection

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