Exosome Characterization using Flow Nano Analyzer

Exosome samples were diluted in PBS (phosphate buffered saline) 1:10–1:100 and analysed using the Flow Nano Analyzer (NanoFCM Inc. (Nottingham, UK)), according to the manufacturer’s protocol [21 (link)]. Briefly, lasers were calibrated using 200 nm control beads (NanoFCM Ltd.), which were analysed as a reference for particle concentration. A mixture of various-sized beads (NanoFCM Inc.) was analysed to set a reference for size distribution. PBS was analysed as a background signal. Particle concentrations and size distributions were calculated using NanoFCM software (NanoFCM profession V1.0) and normalised to the cell number and dilution necessary for adequate NanoFCM reading.

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Jackson H.K., Mitoko C., Linke F., Macarthur D., Kerr I.D, & Coyle B. (2023). Extracellular Vesicles Potentiate Medulloblastoma Metastasis in an EMMPRIN and MMP-2 Dependent Manner. Cancers, 15(9), 2601.

Publication 2023

Flow Nano Analyzer 200 nm control beads

Dilution Exosome Phosphate Saline

Corresponding Organization : University of Nottingham

Other organizations : University of Cambridge, Erasmus MC Cancer Institute, Queen's Medical Centre

Top 5 similar protocols

Variable analysis

independent variables

Dilution of exosome samples in PBS (1:10–1:100)

dependent variables

Particle concentration
Size distribution

control variables

Lasers calibrated using 200 nm control beads
Mixture of various-sized beads analyzed to set a reference for size distribution
PBS analyzed as a background signal

positive controls

200 nm control beads (NanoFCM Ltd.) analyzed as a reference for particle concentration

negative controls

PBS analyzed as a background signal

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