Detergent Preparation and Characterization

The following detergents were commercially available: Triton X-100 (TX-100; Merck (Rahway, NJ, USA) Cat. No. 108643), Triton X-100 reduced (TX-100R; Sigma Aldrich (St. Louis, MO, USA), Cat. No. X100RS), Polysorbate 80 (PS80; Merck, Cat. No. 817061), and Polysorbate 20 (PS20; Merck, Cat. No. 44112). Nereid (4-(1,1,3,3-tetramethyl-butyl)benzyl-polyethylene glycol) is a proprietary compound, synthetized by Takeda as previously described [16 (link)]. Detergent stock solutions were prepared gravimetrically, i.e., by weighing the respective detergent on an analytical scale, followed by addition of the respective matrix to reach the targeted molar concentration of the detergent.