The methicillin resistance of 40 S. aureus isolates were tested by using the Oxacillin agar screen method on the Muller Hinton agar modified by adding NaCl (4 %) + Oxacillin (6 µg/ml) (Dhanalakshmi et al. 2012 ). The strains that could grow on this medium were then tested to be revealed whether they carry the mecA gene (Kot et al. 2020 (link); Ubukata et al. 1989 (link)), as described in the next section.
The disc diffusion method was conducted to determine the antibiotic resistance pattern of the identified methicillin-resistant S. aureus strains to Vancomycin (30 µg), Ciprofloxacin (5 µg), Erythromycin (15 µg), Tetracycline (30 µg), Gentamycin (10 µg), Ceftriaxone (30 µg), Amikacin (30 µg), Mupirocin (5 µg), Oxacillin (30 µg), Chloramphenicol (30 µg) and Trimethoprim/Sulfamethoxazole (1.25/23.75 µg) purchased from MAST Group, Merseyside, UK.
The disc diffusion method was conducted to determine the antibiotic resistance pattern of the identified methicillin-resistant S. aureus strains to Vancomycin (30 µg), Ciprofloxacin (5 µg), Erythromycin (15 µg), Tetracycline (30 µg), Gentamycin (10 µg), Ceftriaxone (30 µg), Amikacin (30 µg), Mupirocin (5 µg), Oxacillin (30 µg), Chloramphenicol (30 µg) and Trimethoprim/Sulfamethoxazole (1.25/23.75 µg) purchased from MAST Group, Merseyside, UK.
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