Blood samples and anthropometrics were collected at two time points: baseline and after a 12-month follow-up. Height (cm), weight (cm), body mass index BMI (kg/m2), waist (cm) and hip (cm) circumferences, Systolic and diastolic blood pressures (SBP and DBP, respectively, measured as the average of 2 readings with a 15-min interval, using pediatric cut-offs appropriate for children’s sizes).
All participants were instructed to come in a 10-h overnight fasting state to their respective schools. Fasting blood samples were collected by trained nurses at baseline and after intervention. Fasting blood glucose, high-density lipoprotein cholesterol (HDL-c), total cholesterol (TC), and triglycerides (TG) were measured using a standard routine laboratory analysis (Konelab, Finland). Low-density lipoprotein cholesterol (LDL-c) was calculated using the Friedwald equation. HbA1c was analyzed using the D-10 Hemoglobin Testing System (ion-exchange chromatography) (Bio-Rad, Hercules, CA, USA). Overweight and obese were classified according to sex, age and BMI percentile for children as done previously [35 (link),36 (link)].
All participants were instructed to come in a 10-h overnight fasting state to their respective schools. Fasting blood samples were collected by trained nurses at baseline and after intervention. Fasting blood glucose, high-density lipoprotein cholesterol (HDL-c), total cholesterol (TC), and triglycerides (TG) were measured using a standard routine laboratory analysis (Konelab, Finland). Low-density lipoprotein cholesterol (LDL-c) was calculated using the Friedwald equation. HbA1c was analyzed using the D-10 Hemoglobin Testing System (ion-exchange chromatography) (Bio-Rad, Hercules, CA, USA). Overweight and obese were classified according to sex, age and BMI percentile for children as done previously [35 (link),36 (link)].
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