Quantifying miR-493-3p in Prostate Cancer

Tissue microarrays (TMAs) were purchased from Xinchao Biotech, Shanghai, China, which contained 81 cases with paired tumor and non-tumor tissues. A 5′-DIG and 3′-DIG-labeled, locked nucleic acid-incorporated miRNA probe (miRCURY LNATM Detection probe, Exiqon, Woburn, MA, USA) was applied for the detection of miR-493-3p in PCa TMAs, The probe sequence of hsa-miR-493-3p was designed as follows: 5’-CCTGGCACACAGTAGACCTTCA -3’. The specific procedures were performed as previously described [34 (link)]. Both the intensity and proportion of positive cells were considered for the semi-quantification of the strength of positivity.