Comprehensive Western Blot Protocol

Western blotting was performed according to a previous report [28 (link)]. The Rabbit anti-VEGF (19003-1-AP), Rabbit anti-FLK1 (26415-1-AP), Rabbit anti-NFDUFS4 (15849-1-AP), Rabbit anti-alpha-SYN (10842-1-AP), Rabbit anti-TH (25859-1-AP), and Rabbit anti-GAPDH (10494-1-AP) primary antibodies were purchased from Proteintech (Wuhan, China). The Rabbit anti-P38MAPK (5140), Rabbit anti-ERK1/2 (4370), Rabbit anti-p-ERK1/2 (4695), Rabbit anti-SMAD3 (9523), Rabbit anti-p-SMAD3 (C25A9), Rabbit anti-caspase3 (9662) and Rabbit anti-ICAM1(4915) primary antibodies were purchased from Cell Signaling Technology (Danvers, USA). The FITC-labeled anti-Rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, USA).

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Xue C., Li X., Ba L., Zhang M., Yang Y., Gao Y., Sun Z., Han Q, & Zhao R.C. (2021). MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease. Aging and Disease, 12(5), 1211-1222.

Publication 2021

Rabbit anti-GAPDH (10494-1-AP) Rabbit anti-caspase3 (9662)

Anti antibodies Anti syn Antibodies Caspase3 Erk1 Fitc Gapdh Icam1 P38mapk Rabbit Smad3 Vegf

Corresponding Organization : Peking Union Medical College Hospital

Top 5 similar protocols

Variable analysis

independent variables

Western blotting technique

dependent variables

NDUFS4
Alpha-SYN
GAPDH
P38MAPK
ERK1/2
P-ERK1/2
SMAD3
P-SMAD3
Caspase3
ICAM1

control variables

GAPDH (loading control)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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