Example 7
Example 7 provides a method which can be used for producing bone graft substitutes of the present invention.
Reagents
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- 0.14M NaF solution
- Absolute (100%) ethanol
- tetraethyl orthosilicate (TEOS, (Si(OC2H5)4))
- Brushite (CaHPO4·2H2O) Brushite is dissolved in 0.14 M solution of NaF, after which ethanol is added. This mixture is then stirred for 5 minutes.
Finally the TEOS is combined slowly with the solution and is allowed to stir for thirty seconds.
4 ml of the solution is cast into cylindrical moulds (Ø11 mm×50 mm height, via syringe). Each mould is then covered with film and placed into glass container.
Each sample is then gelled for 48 hours at 60° C.
Each sample is then placed into 60% ethanol. After 24 hours the solution is changed for 80% ethanol. After another 24 hours it is changed once again for 95% ethanol. Finally the solution is replaced with 100% ethanol.
Each sample is dried using the CPD method using a Tousimis® 931 critical point drier. Each sample is run through three stasis cycles of eight hours each.
After critical drying each sample is then calcined at 700° C. for three hours.
