Example 2
As a non-limiting example, the perfusion chamber or the multi-well plate described herein can be used for measurement of cellular activity. For example, live brain tissue sample can be sectioned from a fresh brain tissue by Vibratome and cultured in a perfusion chamber or a multi-well plate described here. Tissue culture medium specific for live brain samples can be perfused through (or added by pipetting) to maintain tissue viability. Voltage-sensitive dyes can be added to the culture medium and perfused to the perfusion chamber (or added to the multi-well plate by pipetting) to interact with the brain tissue sample. Fast membrane potential changes, e.g., action potentials in single neurons, can be optically recorded by time-lapse fluorescent microscopy. Immediately following the recording, the brain tissue sample can be fixed by 2% paraformaldehyde. Following fixation, the cover and gasket are disassembled. The tissue sample can then be subjected to a spatial analysis workflow as described herein.
