Drosophila Genetics and Developmental Biology

Flies were grown and crossed on standard food containing cornmeal, molasses, and yeast at room temperature (~22°C). The following fly strains were used in this study: Mef2-GAL4 (Bloomington Drosophila Stock Center (BDSC) #27390), UAS-CD8::GFP, UAS-lacZ, UAS-dpp-GFP (BDSC #53716) (Teleman and Cohen, 2000 (link)), dpp-GAL4 (BDSC #1553), Mi{MIC}dpp^MI03752 (BDSC #36399) PBac{RB}e00178 (Exelixis at Harvard Medical School), Pngl^ex14 (Funakoshi et al., 2010 (link)), UAS-Pngl^RNAi KK101641 (Vienna Drosophila Resource Center), UAS-attB-NGLY1^WT-VK31 (Galeone et al., 2017 (link)), UAS-attB-NGLY1^N41P-VK31, UAS-attB-NGLY1^F70A/G80A-VK31, dpp^HA and dpp^HA-3NQ (this study). For survival (eclosion test), the expected ratio of offspring was calculated based on Mendelian inheritance for each genotypic class and the observed/expected ratio is reported as a percentage.

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Galeone A., Adams J.M., Matsuda S., Presa M.F., Pandey A., Han S.Y., Tachida Y., Hirayama H., Vaccari T., Suzuki T., Lutz C.M., Affolter M., Zuberi A, & Jafar-Nejad H. (2020). Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation. eLife, 9, e55596.

Publication 2020

Mef2-GAL4 dpp-GAL4

Drosophila Food Genotypic Inheritance Lacz Molasses Strains Yeast

Corresponding Organization :

Other organizations : University of Milan, Baylor College of Medicine, University of Basel, Jackson Laboratory

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Variable analysis

independent variables

Fly strains used in the study: Mef2-GAL4, UAS-CD8::GFP, UAS-lacZ, UAS-dpp-GFP, dpp-GAL4, Mi{MIC}dpp^MI03752, PBac{RB}e00178, Pngl^ex14, UAS-Pngl^RNAi, UAS-attB-NGLY1^WT-VK31, UAS-attB-NGLY1^N41P-VK31, UAS-attB-NGLY1^F70A/G80A-VK31, dpp^HA, and dpp^HA-3NQ

dependent variables

Survival (eclosion test)

control variables

Standard food containing cornmeal, molasses, and yeast
Room temperature (~22°C)

positive controls

None specified

negative controls

None specified

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