IHC was performed on 4 µm formalin-fixed, paraffin-embedded tissue sections using BRG1 (SMARCA4), AR, FOXA1, ERG and C-Myc. The IHC process was performed on the Ventana ULTRA automated slide staining system using the Omnimap and Ultraview Universal DAB detection kit. The antibody details are provided in SI Appendix, Table S1. The following commercial kits from Roche-Ventana Medical System were used: Discovery CC1 (Cat No. 950-500), Discovery CC2 (Cat No. 950-123), OptiView Universal DAB Detection Kit (Cat No. 760-700), and OmniMap Universal DAB Detection Kit (Cat No. 760-149).
He T., Cheng C., Qiao Y., Cho H., Young E., Mannan R., Mahapatra S., Miner S.J., Zheng Y., Kim N., Zeng V.Z., Wisniewski J.P., Hou S., Jackson B., Cao X., Su F., Wang R., Chang Y., Kuila B., Mukherjee S., Dukare S., Aithal K.B., D.S. S., Abbineni C., Vaishampayan U., Lyssiotis C.A., Parolia A., Xiao L, & Chinnaiyan A.M. (2024). Development of an orally bioavailable mSWI/SNF ATPase degrader and acquired mechanisms of resistance in prostate cancer. Proceedings of the National Academy of Sciences of the United States of America, 121(15), e2322563121.
Detection kits used: Omnimap and Ultraview Universal DAB (Protocol 1), OptiView Universal DAB (Protocol 3), ultraView Universal DAB (Protocol 4), UltraView Universal DAB (Protocol 5)
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
