Flow Cytometric Analysis of NSCLC Stem Cell Markers

Expression of human NSCLC stem cell marker CD133 and CD44 was analyzed by flow cytometric study in A549 cells and spheroids by using anti-CD133-FITC and anti-CD44-APC antibodies (BD Biosciences). CD133⁺ and CD44⁺ CSCs were flow-cytometrically gated from spheroids on the basis of the cell surface phenotype. Mean fluorescence intensities of Oct-4-PerCP-Cy5.5, Nanog-PE, Sox-2- Alexa Fluor-647, Mrp1-FITC, Aldh1-FITC (BD Biosciences) were quantified^{22 (link)}. Mean fluorescence intensities of Akt, p-Akt, Cxcr-4, Mdr1, Integrin-α2, Integrin-α5, Integrin-β1, p-Fak (Santa Cruz Biotechnology, Inc.) were determined with respective primary antibodies conjugated with PE as previously described^{13 (link)}.

Free full text: Click here

Khan P., Bhattacharya A., Sengupta D., Banerjee S., Adhikary A, & Das T. (2019). Aspirin enhances cisplatin sensitivity of resistant non-small cell lung carcinoma stem-like cells by targeting mTOR-Akt axis to repress migration. Scientific Reports, 9, 16913.

Publication 2019

anti-CD44-APC Nanog-PE Sox-2- Alexa Fluor-647 Mrp1-FITC Aldh1-FITC p-Akt Cxcr-4 Integrin-α2 Integrin-α5 Integrin-β1 p-Fak

A549 cells Aldh1 Alexa fluor 647 Anti antibodies Antibodies Cd44 Cell Cxcr 4 Cy5 5 Fitc Flow cytometric Fluorescence Human Integrin Integrin α2 Mrp1 Nsclc Oct 4 Phenotype Sox 2 Stem cell

Corresponding Organization :

Other organizations : Bose Institute

Top 5 similar protocols

Variable analysis

independent variables

Expression of human NSCLC stem cell marker CD133 and CD44

dependent variables

Percentage of CD133+ and CD44+ CSCs
Mean fluorescence intensities of Oct-4, Nanog, Sox-2, Mrp1, Aldh1
Mean fluorescence intensities of Akt, p-Akt, Cxcr-4, Mdr1, Integrin-α2, Integrin-α5, Integrin-β1, p-Fak

control variables

A549 cells and spheroids

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!