Retinal Layer Thickness Measurement via SD-OCT

Spectral-domain (SD) -OCT device (Bioptigen Inc. Durham. NC, USA) was used to record the retinal layers thickness [30 (link), 31 (link)]. Images were captured with the rectangular scan at 1000 A-scans per B-scan, and 100 B-scans per frame. Total retinal thickness (TRT) was measured perpendicularly from the layer of retinal nerve fiber layer (RNFL) to the layer of outer segment (OS), 500 μm away from the center of optic nerve head (ONH) using the built-in software (InVivoVU, Bioptigen) and then averaged. The examiners were blinded to the treatment information.

Free full text: Click here

Wang Y., Ma X., Muthuraman P., Raja A., Jayaraman A., Petrukhin K., Cioffi C.L., Ma J.X, & Moiseyev G. (2022). The novel visual cycle inhibitor (±)-RPE65-61 protects retinal photoreceptors from light-induced degeneration. PLoS ONE, 17(10), e0269437.

Publication 2022

SD) -OCT device InVivoVU

Device Frame Nerve fiber Optic nerve head Retinal Scans

Corresponding Organization : Wake Forest University

Other organizations : Albany College of Pharmacy and Health Sciences, Columbia University, Rensselaer Polytechnic Institute

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total retinal thickness (TRT) measured perpendicularly from the layer of retinal nerve fiber layer (RNFL) to the layer of outer segment (OS), 500 μm away from the center of optic nerve head (ONH)

control variables

Image capture settings: rectangular scan at 1000 A-scans per B-scan, and 100 B-scans per frame
Measurement method: Using the built-in software (InVivoVU, Bioptigen) and then averaged
Examiners were blinded to the treatment information

controls

No positive or negative controls were explicitly mentioned in the input protocol.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!