RNA extraction was performed using the AllPrep DNA/RNA Mini Kit (Qiagen, Venlo, Germany). Libraries for RNA next-generation sequencing were generated by TruSeq mRNA (Illumina) and sequenced on an Illumina NovaSeq 6000 with 2 × 150 bp and 30 Mio read pairs per sample. Reads were aligned to the human genome (Homo sapiens GRCh38) using STAR software v. 2.6 [12 (link),13 (link)].
Mapped reads were counted with HTSeq [13 (link)]. Read counts were normalized with DESeq2 for sequencing depth and RNA composition using the median of ratios method. [14 (link)].
A complete analysis of transcriptomic data will be published elsewhere (manuscript in preparation).
Mapped reads were counted with HTSeq [13 (link)]. Read counts were normalized with DESeq2 for sequencing depth and RNA composition using the median of ratios method. [14 (link)].
A complete analysis of transcriptomic data will be published elsewhere (manuscript in preparation).
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