Genetic mouse models for adiposity studies

Lats1^fl/fl mice (024941, The Jackson Laboratory), Lats2^fl/fl mice^{48 (link)} and Rosa26-LSL-tdTomato mice (007914, The Jackson Laboratory), Lep^ob/+ mice (000632, The Jackson Laboratory), Yap^fl/fl mice^{49 (link)}, Taz^fl/fl mice^{50 (link)}, Adipoq-Cre (010803, The Jackson Laboratory) and Adipoq-CreER^T2 transgenic mice (024671, The Jackson Laboratory) were bred to generate mice used in this study. Tamoxifen (13258, Cayman Chemical) was dissolved in corn oil (C8267, Sigma) and administered via intraperitoneal injection to mice every other day for three doses of 100 mg per kg body weight Tamoxifen to induce CreER^T2 activity. Littermates with control genotypes served as the control group. Male mice were used in all studies except for experiments in Figs. 2c,d and 6e, and Fig. 5, which included both male and female mice. All mice were housed in a specific pathogen-free facility within the Korea Advanced Institute of Science and Technology Laboratory Animal Resource Center. Mice were maintained under a 12-h light–dark cycle and given free access to chow diet (2018, Teklad), chow diet containing 5 mg per kg body weight rosiglitazone (122320-73-4, Adooq Bioscience) or diet containing 60 kcal% fat (D12492, Research Diets) and water. All protocols for mouse experiments were approved by the Institutional Animal Care and Use Committee of the Korea Advanced Institute of Science and Technology.