Culturing Human Neural Progenitor Cells

Human neural progenitor cells (hNPCs) were
generated and cultured
similar to the previously described methods.^{27 (link)} Briefly, hNPCs were cultured on laminin (LN)- or VDP-coated tissue
culture plates in the presence of neural expansion medium [NEM; 1×
DMEM-F12 (Thermo Fisher), 0.5% (v/v) N2 supplement (Thermo Fisher),
1% (v/v) B27 supplement (Thermo Fisher), 1% (v/v) GlutaMAX supplement
(Thermo Fisher), 1% (v/v) Penicillin Streptomycin (Thermo Fisher),
30 ng/mL FGF2 (STEMCELL Technologies), and 30 ng/mL EGF (STEMCELL
Technologies)]. Every 3–4 days, hNPCs were enzymatically passaged
with Accutase (Thermo Fisher) onto freshly coated LN or VDP plates.

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Raman S., Srinivasan G., Brookhouser N., Nguyen T., Henson T., Morgan D., Cutts J, & Brafman D.A. (2020). A Defined and Scalable Peptide-Based Platform for the Generation of Human Pluripotent Stem Cell-Derived Astrocytes. ACS Biomaterials Science & Engineering, 6(6), 3477-3490.

Publication 2020

DMEM-F12 N2 supplement B27 supplement GlutaMAX supplement Penicillin Streptomycin FGF2 EGF Accutase

Accutase Fgf2 Human Laminin Neural plates Neural progenitor cells Penicillin Stemcell Streptomycin Supplement

Corresponding Organization : Arizona State University

Other organizations : University of Arizona

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Variable analysis

independent variables

Coating of tissue culture plates with laminin (LN) or VDP

dependent variables

Growth and proliferation of human neural progenitor cells (hNPCs)

control variables

Neural expansion medium (NEM) composition: 1× DMEM-F12, 0.5% (v/v) N2 supplement, 1% (v/v) B27 supplement, 1% (v/v) GlutaMAX supplement, 1% (v/v) Penicillin Streptomycin, 30 ng/mL FGF2, and 30 ng/mL EGF
Passaging of hNPCs every 3-4 days with Accutase onto freshly coated LN or VDP plates

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