The activity of caspase-3 and PARP in HeLa, HEC-1-A, and KLE cancer cells were developed as previously reported (Zhang and Snyder, 1992 (link); Kang et al., 2012 (link)). Various siRNA sequences from the LKB1 RNA sequence were prepared utilizing a siRNA Construction kit (Ambion, Austin, TX, USA). After selection of two clones, the prepared sequences were then transfected with oligofectamine (Thermo Fisher Scientific, Carlsbad, CA, USA) in accordance with supplier’s recommendation. TRIZOL Reagent (Thermo Fisher Scientific) was used to isolate whole RNA and reverse transcription (RT) PCR was then conducted. LKB1 expression was measured via western blot analysis.
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Caspase-3 and PARP Activity in Cancer Cells
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Austin
Cancer
Caspase 3
Cells
Clones
Hela
Lkb1
Oligofectamine
Reverse transcription
Rna sequences
Sirna
Trizol
Western blot analysis
Corresponding Organization :
Other organizations : National Cancer Center, Dongguk University
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Variable analysis
independent variables
- SiRNA sequences from the LKB1 RNA sequence
- The activity of caspase-3 and PARP in HeLa, HEC-1-A, and KLE cancer cells
- LKB1 expression
- Not explicitly mentioned
- Positive controls: Not mentioned
- Negative controls: Not mentioned
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