Veratridine and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX) were purchased from Tocris Bioscience (UK). Veratridine (50 μM) [31 (link),83 (link)] and NBQX (100 μM) [84 (link),85 (link)], were infused through the intracerebral probe into the DRN of the animals.
[33P]α-dATP (2′ deoxyadenosine 5′-(α-thio) triphosphate at a specific activity of >2500 Ci/mmol was purchased from Perkin Elmer and GTPγS (10 μM) and used for the mTOR and BDNF in situ hybridization studies.
[33P]α-dATP (2′ deoxyadenosine 5′-(α-thio) triphosphate at a specific activity of >2500 Ci/mmol was purchased from Perkin Elmer and GTPγS (10 μM) and used for the mTOR and BDNF in situ hybridization studies.
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