Melatonin Mitigates Myocardial Infarction

The mice were randomly divided into three groups: the sham group (n = 12), the MI group (MI, n = 12), and the melatonin treatment group (MI+MLT, n = 12). The MI model was generated with a method similar to that in previous studies [23 (link), 24 (link)]. Briefly, the mice were anaesthetized by avertin (0.2 g/kg) injection. The left anterior descending coronary artery was exposed and ligated with a 7-0 nylon suture for 24 h. The mice in the sham group underwent the same surgical procedures without coronary artery ligation. The mice in the MI+MLT group were administered 10 mg/kg melatonin (M5250, Sigma-Aldrich) per day by intragastric gavage for 14 days prior to MI surgery [25 (link)]. The mice in the sham and MI groups were given an equivalent volume of ddH₂O.