Drosophila Behavioral Assays and Genetic Lines

Flies were reared on regular cornmeal diet and housed in standard conditions. The following laboratory fly lines were used: TubP-Gal4, nSyb-Gal4, OK107-Gal4, MB247-Gal4, TubP-Gal80^ts; nSyb-Gal4. We obtained the following fly lines from Bloomington Drosophila Stock Center: w¹¹¹⁸ (BDSC 3605), NPF-Gal4 (BDSC 25681), TH-Gal4 (BDSC 8848), Gr5a-Gal4 (BDSC 57591), Gr66a-Gal4 (BDSC 57670), UAS-shi^ts (BDSC 44222), Rut²⁰⁸⁰ (BDSC 9405), Dnc¹ (BDSC 6020), UAS-HTT128Q^FL (BDSC 33808), and UAS-miR-rCD2^{42 (link),43 (link)}. Gr43a^Gal4 was obtained from Dr. Hubert Amrein, Texas A&M University^{9 (link)}. GMR-Gal4, UAS-41Q-HA, and UAS-41Q-HA were obtained from Dr. Horng-Dar Wang, National Tsing Hua University, Taiwan^{44 (link)}. Elav-GeneSwitch (ElavGS) was obtained from Dr. Chun-Hong Chen, National Health Research Institutes. In most cases, flies were reared at 25 °C. For the Gal4/UAS-shi^ts experiments, flies were reared at 23 °C until 1 h before the VBFD assay (32 °C). To express UAS-41Q-HA in adult flies via the TARGET system¹⁷, animals were allowed to develop at 18 °C and transferred to 29 °C right after eclosion.

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Yu C.C., Chang F.C., Hong Y.H., Li J.C., Chen P.L., Chen C.H., Chiu T.W., Lu T.T., Wang Y.M, & Kao C.F. (2021). Assessing the cognitive status of Drosophila by the value-based feeding decision. NPJ Aging and Mechanisms of Disease, 7, 24.

Publication 2021

w1118 NPF-Gal4 TH-Gal4 Gr5a-Gal4 Gr66a-Gal4 UAS-shits Rut2080

Adult Animals Assay Center lines Drosophila Flies Regular diet

Corresponding Organization :

Other organizations : National Yang Ming Chiao Tung University, National Tsing Hua University, National Health Research Institutes

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Variable analysis

independent variables

Gal4 drivers (TubP-Gal4, nSyb-Gal4, OK107-Gal4, MB247-Gal4, NPF-Gal4, TH-Gal4, Gr5a-Gal4, Gr66a-Gal4, Gr43a-Gal4, GMR-Gal4)
UAS effectors (UAS-shi^ts, UAS-HTT128Q^FL, UAS-miR-rCD2, UAS-41Q-HA)
Temperature (25 °C, 23 °C, 32 °C, 18 °C, 29 °C)

dependent variables

Fly behavior and physiology (not explicitly specified)

control variables

Fly rearing conditions (regular cornmeal diet, standard housing conditions)
Fly lines (w^1118, Rut^2080, Dnc^1)

positive controls

W^1118 (BDSC 3605)
Rut^2080 (BDSC 9405)
Dnc^1 (BDSC 6020)

negative controls

Not explicitly mentioned

Annotations

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