Insulin, GLP-1, and Intralipid + heparin were infused for various times as detailed in each study protocol. Insulin (Humulin; Eli Lilly and Company) was infused systemically at 3 mU/kg/min with arterial blood glucose determined every 10 min using an Accu-Chek Advantage glucometer (Roche Diagnostics, Indianapolis, IN) and 30% dextrose (30% weight for volume) infused at a variable rate to maintain blood glucose within 10% of basal. The time course and area under the curve (AUC) of the glucose infusion rate (GIR; mg/kg/min) were calculated. GLP-1 (7–36) amide (Bachem Americas, Inc.) was infused continuously at 30 pmol/kg/min. Intralipid (6.6%) plus heparin (60 U/mL) was infused at 5 µl/min. At the rates selected, insulin and GLP-1 each potently recruit muscle microvasculature (8 (link),11 (link),23 (link),24 (link)), and Intralipid + heparin abrogates insulin-mediated muscle microvascular and metabolic responses (8 (link),33 (link)).
Protocol detail
Insulin, GLP-1, and Intralipid Interaction
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Amide
Arterial
Blood glucose
Diagnostics
Glp 1
Glucose
Heparin
Humulin
Insulin
Intralipid
Microvasculature
Muscle
Corresponding Organization :
Other organizations : University of Virginia Health System, Xiangya Hospital Central South University, Central South University
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Variable analysis
independent variables
- Insulin (Humulin; Eli Lilly and Company) infusion rate
- GLP-1 (7–36) amide (Bachem Americas, Inc.) infusion rate
- Intralipid (6.6%) plus heparin (60 U/mL) infusion rate
- Time course and area under the curve (AUC) of the glucose infusion rate (GIR; mg/kg/min)
- Arterial blood glucose determined every 10 min using an Accu-Chek Advantage glucometer (Roche Diagnostics, Indianapolis, IN)
- 30% dextrose (30% weight for volume) infused at a variable rate to maintain blood glucose within 10% of basal
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