To assess the binding of serum antibodies from weeks 0, 4, 6, 12, 22, 36, and 38 against the AMC009, AMC011, and AMC008 SOSIP trimers, the purified His-tagged trimers were immobilized on Ni-NTA ELISA plates (Qiagen) (80 (link)). Subsequent steps are the same as those for D7324 capture ELISA.
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