All animals were on the C57BL/6 genetic background unless otherwise specified. HuLangerin-Cre I-Aβ (10 (link)), huLangerin-Cre MyD88(6 (link)), huLangerin-DTA (11 (link)), mice have all been previously described. Rag1−/−, IL-6−/−, IL-1β−/−, MyD88/TRIF−/− and B6.129P2-Cxcr6tm1Litt were purchased from Jackson Labs (Bar Harbor, ME). IL-23−/− mice were a kind gift from Nico Ghilardi (Genentech, San Francisco CA). NLRP3−/− and ASC−/− mice were acquired from Millenium Pharmacerticals(12 (link)). All experiments were performed with 8–10 week old age- and sex-matched littermates. Mice were housed in specific pathogen free microisolator cages and fed irradiated food and acidified water. The University of Minnesota institutional animal care and use committee approved all animal protocols.
Protocol detail
Genetic Knockout Mouse Models
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Animal
Genetic background
Il 1β
Institutional animal care and use committee
Irradiated food
Mice
Rag1
Specific pathogen free
Corresponding Organization : University of Minnesota Medical Center
Other organizations : Rockefeller University, Iowa City VA Medical Center, University of Iowa
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Variable analysis
independent variables
- Genotypes: HuLangerin-Cre I-Aβ, huLangerin-Cre MyD88, huLangerin-DTA, Rag1−/−, IL-6−/−, IL-1β−/−, MyD88/TRIF−/−, B6.129P2-Cxcr6tm1Litt, IL-23−/−, NLRP3−/−, ASC−/−
- Not explicitly mentioned
- Age: 8-10 weeks
- Sex: age- and sex-matched littermates
- Genetic background: C57BL/6, unless otherwise specified
- Housing: specific pathogen free microisolator cages
- Diet: irradiated food and acidified water
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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