Human prostate tumor cell lines PC-3 and DU-145, and a mouse prostate tumor cell line TRAMP-C2 were obtained from ATCC (Manassas, VA). PC-3-luc cell line was kindly provided by Kenneth Pienta (University of Michigan, Ann Arbor, MI). All prostate tumor cell lines were maintained in RPMI-1640 media containing 10% fetal calf serum. To create Ad.dcn, the decorin gene was cloned in a shuttle vector and subjected to homologous recombination with adenoviral genomic DNA derived from adenoviral mutant dl01/07, using published methods.41 (link) To create Ad(E1-).dcn, an AdEasy system was used for homologous recombination.42 (link) Ad(E1-).null (the non-replicating adenovirus without any foreign trangene), Ad(E1-).luc (the non-replicating adenovirus containing firefly luciferase2 gene), and Ad.luc (the conditionally replicating adenovirus containing firefly luciferase2 gene) have been previously described.33 (link), 43 (link), 44 (link) All adenoviral vectors were amplified in HEK293 cells (ATCC), and purified as described earlier.43 (link)
Protocol detail
Prostate Cancer Cell Lines and Adenoviral Vectors
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Adenoviral
Adenovirus
Decorin
Fetal calf serum
Firefly
Gene
Genomic
Hek293 cells
Homologous recombination
Human
Mouse
Pc 3 cell line
Prostate
Replicating gene
Shuttle vector
Tumor cell lines
Vectors
Corresponding Organization :
Other organizations : NorthShore University HealthSystem, Sidney Kimmel Cancer Center, Thomas Jefferson University, National Institutes for Food and Drug Control, Argonne National Laboratory, Northwestern University
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Variable analysis
independent variables
- Prostate tumor cell lines (PC-3, DU-145, TRAMP-C2, PC-3-luc)
- Not explicitly mentioned
- RPMI-1640 media containing 10% fetal calf serum
- HEK293 cells used for amplification and purification of adenoviral vectors
- Ad(E1-).null (the non-replicating adenovirus without any foreign trangene)
- Ad(E1-).luc (the non-replicating adenovirus containing firefly luciferase2 gene)
- Ad.luc (the conditionally replicating adenovirus containing firefly luciferase2 gene)
- None explicitly mentioned
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