Fourteen-week-old Dio mice, which had already been given a high fat diet (D12492) for 8 weeks, were purchased from JAX Lab (stock #000664). After quarantine, the mice were divided into three groups. Each group had 8 mice. For 2 weeks, 10 µg/kg body weight/day of HDAC3 inhibitor was administrated by intraperitoneal injection. Control groups were given PBS with 0.1% DMSO (solvent). Pioglitazone was applied in the diet at the dose of 10 mg/kg body weight/day. ITT was conducted by intraperitoneal injection of insulin (I9278, Sigma) at 0.75 unit/kg of body weight in mice after a 4-h fast, as described elsewhere (Gao et al. 2009 (link)). Blood glucose was monitored in the tail vein blood using the FreeStyle blood glucose monitoring system (TheraSense, Phoenix, AZ).
Protocol detail
Metabolic Intervention in Dio Mice
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Blood
Blood glucose
Blood system
Body weight
Diet
Dmso
Glucose
High fat diet
Insulin
Intraperitoneal injection
Mice
Pioglitazone
Quarantine
Solvent
Tail
Vein
Corresponding Organization :
Other organizations : Sun Yat-sen University
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Variable analysis
independent variables
- HDAC3 inhibitor dose (10 μg/kg body weight/day)
- Pioglitazone dose (10 mg/kg body weight/day)
- Blood glucose levels
- Fourteen-week-old Dio mice
- High-fat diet (D12492) for 8 weeks prior to experiment
- Quarantine period after purchase
- Intraperitoneal injection of insulin (0.75 unit/kg of body weight) after 4-h fast
- Blood glucose monitoring using FreeStyle blood glucose monitoring system
- Positive control: Pioglitazone group
- Negative control: PBS with 0.1% DMSO group
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