Unless otherwise noted, all chemicals and reagents were purchased from Sigma Aldrich (St. Louis, MO). Details for all antibodies used in this work are listed in Table 1 .
Briefly, polyclonal antibodies were generated to AnkG from amino acids TEDK to KKTH, to βIV Spectrin from amino acids ARRA to QESA, and to pan-NaV channels from amino acids FNQQ to AFDI of the sodium channel NaV1.6. Other antibodies used are anti-Caspr (Bhat et al., 2001 (link)), anti-NF186 (Thaxton et al., 2011 (link)), and anti-NFCT (Pillai et al., 2009 (link)). Commercial antibodies used included rabbit anti-myelin basic protein (Abcam; Cambridge, MA); mouse anti-α-tubulin (DSHB; Iowa City, IA); mouse anti-Caspr, anti-AnkR and anti-Kv1.2 (NeuroMab; Davis, CA), fluorescent secondary antibodies (Alexa Fluor; Life Technologies; Grand Island, NY), and infrared (IR) conjugated secondary antibodies (LI-COR; Lincoln, NE). All electron microscopy reagents were purchased from Electron Microscopy Sciences (Hatfield, PA).
Briefly, polyclonal antibodies were generated to AnkG from amino acids TEDK to KKTH, to βIV Spectrin from amino acids ARRA to QESA, and to pan-NaV channels from amino acids FNQQ to AFDI of the sodium channel NaV1.6. Other antibodies used are anti-Caspr (Bhat et al., 2001 (link)), anti-NF186 (Thaxton et al., 2011 (link)), and anti-NFCT (Pillai et al., 2009 (link)). Commercial antibodies used included rabbit anti-myelin basic protein (Abcam; Cambridge, MA); mouse anti-α-tubulin (DSHB; Iowa City, IA); mouse anti-Caspr, anti-AnkR and anti-Kv1.2 (NeuroMab; Davis, CA), fluorescent secondary antibodies (Alexa Fluor; Life Technologies; Grand Island, NY), and infrared (IR) conjugated secondary antibodies (LI-COR; Lincoln, NE). All electron microscopy reagents were purchased from Electron Microscopy Sciences (Hatfield, PA).
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