Photoconvertible Fluorescent Protein Fusions

PA-FP protein fusions were constructed with green-to-red photoconvertible fluorescent protein, tandemdimer Eos (tdEos)19 (link) or monomeric Eos2 (mEos2)20 (link) fused to focal adhesion proteins via short linkers (Supplementary Table 3 and Supplementary Note 3). Fusion proteins were expressed in U2OS or MEF cells sparsely plated on fibronectin-coated, fiducialed coverglasses, and fixed for imaging ~18 h after re-plating.

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Kanchanawong P., Shtengel G., Pasapera A.M., Ramko E.B., Davidson M.W., Hess H.F, & Waterman C.M. (2010). Nanoscale architecture of integrin-based cell adhesions. Nature, 468(7323), 580-584.

Publication 2010

Cells Fibronectin Focal adhesion Green fluorescent protein Proteins Red fluorescent protein

Corresponding Organization :

Other organizations : National Heart Lung and Blood Institute, National Institutes of Health, Howard Hughes Medical Institute, Janelia Research Campus, National High Magnetic Field Laboratory, Florida State University

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Variable analysis

independent variables

Type of photoconvertible fluorescent protein fused to focal adhesion proteins (tdEos or mEos2)

dependent variables

Localization and dynamics of focal adhesion proteins in U2OS or MEF cells

control variables

Cell type (U2OS or MEF cells)
Extracellular matrix coating (fibronectin-coated)
Imaging timepoint (~18 h after re-plating)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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