Quantifying Airway Cell Populations

Western blotting and immunohistochemical (fluorescence) staining analyses were performed as described previously18 (link),31 (link) with the following primary antibodies: actin monoclonal (1:5000 dilution; Sigma–Aldrich), FITC-conjugated anti-goat IgG, rhodamine-conjugated anti-rabbit IgG, alkaline phosphatase-conjugated anti-rabbit IgG (Jackson ImmunoResearch Laboratories), AhR and CC10 goat polyclonal antibodies (Santa Cruz Biotechnology), Notch1 and Hes5 rabbit polyclonal antibodies (cell signaling technology), AhR monoclonal antibody (Enzo life Sciences), and GFP rabbit polyclonal antibody (Abcam). Percentages of CC10 and Notch1 or HES5 double-positive cells in total airway cells were counted on 3–6 random ﬁelds from three animals.

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Liu K.Y., Wang L.T., Wang H.C., Wang S.N., Tseng L.W., Chai C.Y., Chiou S.S., Huang S.K, & Hsu S.H. (2021). Aryl Hydrocarbon Receptor is Essential in the Control of Lung Club Cell Homeostasis. Journal of Inflammation Research, 14, 299-311.

Publication 2021

FITC-conjugated anti-goat IgG rhodamine-conjugated anti-rabbit IgG alkaline phosphatase-conjugated anti-rabbit IgG

Actin Alkaline phosphatase Animals Anti igg Antibodies Antibody Cells Dilution Fitc Fluorescence Goat Monoclonal antibody Rabbit Rhodamine

Corresponding Organization :

Other organizations : Shenzhen Third People’s Hospital, Shenzhen University, National Taiwan Normal University, China Medical University, China Medical University Hospital, Kaohsiung Medical University, Kaohsiung Medical University Chung-Ho Memorial Hospital, National Health Research Institutes

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