Live-cell imaging of neuronal cells

Phase and wide-field epifluorescence imaging of live (dissociated or ganglion explants) or fixed cells was performed on Zeiss Axiovert inverted microscopes equipped with a heated stage for live-cell imaging (Ketschek and Gallo, 2010 (link)). For all live-cell imaging, cultures are allowed to equilibrate for 15 min on the heated stage prior to imaging. An Orca ER camera (Hamamatsu) was used for all wide-field image acquisition.

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Sainath R, & Gallo G. (2014). The Dynein Inhibitor Ciliobrevin D Inhibits the Bi-directional Transport of Organelles along Sensory Axons and Impairs NGF-Mediated Regulation of Growth Cones and Axon Branches. Developmental neurobiology, 75(7), 757-777.

Publication 2014

Orca ER camera

Cell Cell cultures Ganglion Microscopes Orca

Corresponding Organization :

Other organizations : Temple University

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Variable analysis

independent variables

Phase and wide-field epifluorescence imaging

dependent variables

Measured outcomes

control variables

Heated stage for live-cell imaging
Allowed 15 min equilibration on heated stage prior to imaging
Orca ER camera (Hamamatsu) for wide-field image acquisition

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