Immunohistochemical Profiling of Tissues

All collected tissues were sectioned and stained with hematoxylin and eosin for histological evaluation. For IHC staining to detect specific antigens, the following antibodies were used: anti-Ki67 (Neomarkers), anti–cleaved caspase-3 (clone 5A1E, Cell Signaling), anti-CD8 (BD Biosciences), anti-NK1.1 (PK136, eBioscience), anti-SV40Tag (Santa Cruz), anti–arginase I (Santa Cruz), and anti-p63 (Neomarkers). The IHC staining protocol has been previously described (39 (link), 42 (link)). All sections were counterstained with hematoxylin for nucleus visualization.

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Zhang J., Liu D., Li G., Staveley-O’Carroll K.F., Graff J.N., Li Z, & Wu J.D. (2017). Antibody-mediated neutralization of soluble MIC significantly enhances CTLA4 blockade therapy. Science Advances, 3(5), e1602133.

Publication 2017

anti–cleaved caspase-3 (clone 5A1E, anti-CD8 anti-NK1.1 (PK136, anti-SV40Tag anti–arginase I

Antibodies Antigens Arginase Caspase 3 Clone Eosin Hematoxylin Nucleus Tissues

Corresponding Organization :

Other organizations : Medical University of South Carolina, University of Missouri, Oregon Health & Science University, MUSC Hollings Cancer Center

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Variable analysis

independent variables

Antibodies used for IHC staining

dependent variables

Expression of specific antigens (Ki67, cleaved caspase-3, CD8, NK1.1, SV40Tag, arginase I, p63)

control variables

Tissue sectioning and staining with hematoxylin and eosin for histological evaluation
Counterstaining of all sections with hematoxylin for nucleus visualization

positive controls

None specified

negative controls

None specified

Annotations

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