Magnetic resonance imaging (MRI) was performed using a high-field magnetic resonance tomograph (Agilent Technologies DD2–400 9.4 T 400 MHz; Cheadle, UK) with a volume coil M2M (H1). The mice were anesthetized intraperitoneally with 70 mg/kg Zoletil 100 (Virbac Sante Animale, Carros, France) and intramuscularly with 0.02 mg/kg Xylanite (NITA-Pharm, Saratov, Russia) and then fixed in an upright position inside the magnet tunnel set at 27 °C.
The VnmrJ software was used for brain scanning and data processing. T1 tomograms of the layered frontal brain sections weighted by proton density were obtained using the multi-gradient echo multi slice (MGEMS) pulse sequence with the following parameters: TR = 1000 ms; TE = 1.49 ms; 6 echoes; FOV—20 × 20 mm; matrix—256 × 256; 15 slices; 1 mm slice thickness; 17 min; and 4 s scanning time [21 (link)].
The VnmrJ software was used for brain scanning and data processing. T1 tomograms of the layered frontal brain sections weighted by proton density were obtained using the multi-gradient echo multi slice (MGEMS) pulse sequence with the following parameters: TR = 1000 ms; TE = 1.49 ms; 6 echoes; FOV—20 × 20 mm; matrix—256 × 256; 15 slices; 1 mm slice thickness; 17 min; and 4 s scanning time [21 (link)].
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