Pharmacological studies of GPCRs generally utilize mammalian cells to avoid the presence of endogenous GPCRs and achieve higher expression efficiency on the cell membrane. HEK293 and its derivative cell lines, such as HEK293T, as well as CHO cells, are the most commonly used cell lines in this field of research [19 (link),22 (link),26 (link),38 (link)]. In this study, we employed HEK293T cells as the research platform according to a method we established previously [39 (link)].
Human embryonic kidney cells HEK293T obtained from Beyotime Biotechnology (Shanghai, China) were cultured with Dulbecco’s Modified Eagle Medium (DMEM) containing a 10% fetal bovine serum in an incubator (37 °C and 5% CO2 humidified atmosphere). The cells were plated into 24-well plates and cultured for 24 h before assays. The Countess 3 cell counter (Invitrogen, Waltham, MA, USA) was used for cell counting.
Human embryonic kidney cells HEK293T obtained from Beyotime Biotechnology (Shanghai, China) were cultured with Dulbecco’s Modified Eagle Medium (DMEM) containing a 10% fetal bovine serum in an incubator (37 °C and 5% CO2 humidified atmosphere). The cells were plated into 24-well plates and cultured for 24 h before assays. The Countess 3 cell counter (Invitrogen, Waltham, MA, USA) was used for cell counting.
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