Transcriptome Profiling by RNA-Seq

Total RNA was extracted as previously reported [43 (link)]. Sample quality and quantity were checked with a Bioanalyzer and RNA 6000 Nano Kit (Agilent Technologies), and a Qubit with the RNA BR Assay Kit (Invitrogen). Sequence libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) and manufacturer’s instructions. Library quality and quantity were checked with a Bioanalyzer and the High Sensitivity DNA Analysis Kit (Agilent Technologies) and a Qubit with the ds DNA HS Assay Kit (Invitrogen). Samples were sequenced on a HiSeq1500 (Illumina) using 50 bp single-end sequencing in rapid-run mode. The dataset is available at the NCBI’s Gene Expression Omnibus under the accession code GSE101312.