The levels of cardiac NOX1, MMP2, and TNF-α were estimated in the supernatants of the rat ventricular tissue homogenates using rat ELISA kits for TNF-α (RAB0480, Sigma-Aldrich, St. Louis, MO, USA), MMP2 (GWB-ZZD134, Genway Biotech, San Diego, CA, USA), and NOX1 (MBS2702015, MyBiosource, Inc., San Diego, CA, USA) according to the manufacturers’ instructions [68 (link)].
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