Apoptosis Analysis of T Cells

Flow cytometry analysis of T cell apoptosis was performed using the Annexin V-PE kit (BD Biosciences, San Jose, California, USA) as previously described (27 (link)). Briefly, T cells were incubated with various concentrations of rHcABHD proteins (0, 10, 20, 40, and 80 μg/mL) followed by staining with annexin V and 7-Aminoactinomycin D (7-AAD) based on the manufacturer's protocol. The PBS-treated goat T cells were served as negative controls. Three independent experiments were performed and each experiment was run in triplicate.

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Lu M., Tian X., Tian A.L., Li C., Yan R., Xu L., Song X, & Li X. (2020). A Novel α/β Hydrolase Domain Protein Derived From Haemonchus contortus Acts at the Parasite-Host Interface. Frontiers in Immunology, 11, 1388.

Publication 2020

Annexin V-PE kit

7 aad Annexin v Apoptosis Flow cytometry Goat Proteins T cells

Corresponding Organization : Nanjing Agricultural University

Other organizations : Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Beltsville Agricultural Research Center, Agricultural Research Service

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Variable analysis

independent variables

Concentrations of rHcABHD proteins (0, 10, 20, 40, and 80 μg/mL)

dependent variables

Apoptosis of T cells

control variables

Incubation time and conditions

controls

Negative control: PBS-treated goat T cells

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