Immunostaining of FOXP3 and MTA1 in Breast Cancer

A total of 37 normal breast and 184 breast cancer tissue specimens were obtained from the Department of Pathology, The First Affiliated Hospital of the Fourth Military Medical University (FMMU, Shaanxi, China). Clinical staging of breast cancer was performed based on the American Joint Committee on Cancer (AJCC) staging system. Clinicopathological data were obtained from the patients’ medical records. This study was approved by our institutional research ethics committee. All specimens were immunostained for FOXP3 and MTA1 as previously described using an antibody against FOXP3 (ab10901, Abcam, 1:200 dilution) or MTA1 (#5647, CST, 1:50 dilution) (20 (link)). Sections of stained breast cancer tissue were sent to Google Biology for observation and scanning.

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Liu C., Han J., Li X., Huang T., Gao Y., Wang B., Zhang K., Wang S., Zhang W., Li W., Hao Q., Li M., Zhang Y, & Zhang C. (2021). FOXP3 Inhibits the Metastasis of Breast Cancer by Downregulating the Expression of MTA1. Frontiers in Oncology, 11, 656190.

Publication 2021

ab10901

Antibody Breast Cancer of breast Dilution Institutional ethics committee Joint Military Mta1 Patients Tissue

Corresponding Organization : Air Force Medical University

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Variable analysis

independent variables

FOXP3 immunostaining
MTA1 immunostaining

dependent variables

Classification of breast tissue samples (normal breast and breast cancer)

control variables

Clinicopathological data of breast cancer patients
Institutional research ethics committee approval

controls

Positive control: Immunostaining of FOXP3 and MTA1 as previously described
Negative control: Not explicitly mentioned

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