Protocol detail

Co-Immunoprecipitation Protocol for APP

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Co-IP was performed as previously described (18 (link)). The following antibodies were used: anti-HA (Sigma-Aldrich, H6908, 1:200), anti-His (Proteintech, 66005-1-Ig, 1:200), isotype rabbit IgG (Santa Cruz Biotechnology, sc-3888, 1:200), and isotype mouse IgG (Santa Cruz Biotechnology, sc-2025, 1:200). A previously described anti-APP rabbit polyclonal antibody RU-369 (1:200; ref. 42 (link)) was used.

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Zheng Q., Song B., Li G., Cai F., Wu M., Zhao Y., Jiang L., Guo T., Shen M., Hou H., Zhou Y., Zhao Y., Di A., Zhang L., Zeng F., Zhang X.F., Luo H., Zhang X., Zhang H., Zeng Z., Huang T.Y., Dong C., Qing H., Zhang Y., Zhang Q., Wang X., Wu Y., Xu H., Song W, & Wang X. (2022). USP25 inhibition ameliorates Alzheimer’s pathology through the regulation of APP processing and Aβ generation. The Journal of Clinical Investigation, 132(5), e152170.

Publication 2022

anti-HA anti-His isotype rabbit IgG sc-3888 sc-2025

Anti antibody Antibodies Isotype Mouse Rabbit

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Variable analysis

independent variables

Anti-HA antibody (Sigma-Aldrich, H6908, 1:200)
Anti-His antibody (Proteintech, 66005-1-Ig, 1:200)
Isotype rabbit IgG (Santa Cruz Biotechnology, sc-3888, 1:200)
Isotype mouse IgG (Santa Cruz Biotechnology, sc-2025, 1:200)
Anti-APP rabbit polyclonal antibody RU-369 (1:200)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Isotype rabbit IgG (Santa Cruz Biotechnology, sc-3888, 1:200)
Isotype mouse IgG (Santa Cruz Biotechnology, sc-2025, 1:200)

Annotations

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