Lentiviral transduction of cell lines

BT474, MDA-MB-361, and T47D cells were transduced with an expression cassette encoding Gluc and GFP separated by an internal ribosomal entry site, using a lentiviral vector as previously described (43 (link)). All cell lines, purchased from the American Type Culture Collection, were authenticated (Cell Check). BT474-Gluc and T47D-Gluc cells were maintained in RPMI 1640 supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals). MDA-MB-361-Gluc cells were maintained in Dulbecco’s modified Eagle’s medium/F12 supplemented with 10% (v/v) FBS.

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Kodack D.P., Askoxylakis V., Ferraro G.B., Sheng Q., Badeaux M., Goel S., Qi X., Shankaraiah R., Cao Z.A., Ramjiawan R.R., Bezwada D., Patel B., Song Y., Costa C., Naxerova K., Wong C.S., Kloepper J., Das R., Tam A., Tanboon J., Duda D.G., Miller C.R., Siegel M.B., Anders C.K., Sanders M., Estrada M.V., Schlegel R., Arteaga C.L., Brachtel E., Huang A., Fukumura D., Engelman J.A, & Jain R.K. (2017). The brain microenvironment mediates resistance in luminal breast cancer to PI3K inhibition through HER3 activation. Science translational medicine, 9(391), eaal4682.

Publication 2017

RPMI 1640 fetal bovine serum

Biologicals Cell lines Cells Eagle Fetal bovine serum Internal ribosomal entry site Vector

Corresponding Organization : Harvard University

Other organizations : University of North Carolina at Chapel Hill, Vanderbilt University, Breast Cancer Research Foundation

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Variable analysis

independent variables

Transduction of BT474, MDA-MB-361, and T47D cells with an expression cassette encoding Gluc and GFP separated by an internal ribosomal entry site, using a lentiviral vector

dependent variables

Not explicitly mentioned

control variables

Cell lines were purchased from the American Type Culture Collection and authenticated (Cell Check)
BT474-Gluc and T47D-Gluc cells were maintained in RPMI 1640 supplemented with 10% (v/v) fetal bovine serum (FBS) (Atlanta Biologicals)
MDA-MB-361-Gluc cells were maintained in Dulbecco's modified Eagle's medium/F12 supplemented with 10% (v/v) FBS

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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