The relative proportion of SMA+ and SR+ areas was measured. For quantification of SMA-positive cells, it is common to analyze the stained areas [62 (link),63 (link)], as a reliable assignment to individual cells is not possible.
Quantifying Arterial Plaque Composition
The relative proportion of SMA+ and SR+ areas was measured. For quantification of SMA-positive cells, it is common to analyze the stained areas [62 (link),63 (link)], as a reliable assignment to individual cells is not possible.
Corresponding Organization : RWTH Aachen University
Variable analysis
- Staining method (Direct Red 80, ki67 antibody M7248, SMA antibody M0851)
- Relative proportion of SMA+ and SR+ areas
- Overall cell number in ki67-stained sections
- Cell density per mm² in ki67-stained sections
- Number of ki67+ cells per cross-section
- Threshold for positive cell detection using pixel intensity histogram
- Manual designation of media and neointima areas prior to automated quantification
- No positive or negative controls were explicitly mentioned.
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