Cell-type Specific Anterograde Viral Tracing

For cell type-specific anterograde viral tracing AAV5.EF1a.DIO.eYFP.WPRE.hGH (30–100 nl; Penn Vector Core; #27056-AAV5; titer: 5 × 10¹² GC/ml) or AAV5-EF1a-DIO-mCherry viruses (30–100 nl; UNC Vector Core; #50462; titer: 7 × 10¹² GC/ml) were injected at a rate of 0.5–1 nl/s into mPFC (AP/L/DV: +1.7–1.9/±0.3/2.1–1.6 mm) and M1 (AP/L/DV: +1.4/±1.6/1.3–1.0 mm) using a Nanoliter Injector (World Precision Instruments, FL, USA).
Animals were perfused (see above) after 4–6 weeks of survival time. Viral expression was always analyzed after IHC_Fluo enhancement (Figure 3—figure supplement 1; Falcy et al., 2020 (link)), even for eYFP (see below).