Transfection of HEK293 Cells with pEGFP-mTRPM7

HEK293 cells were chemically transfected with pEGFP-mTRPM7 and pEGFP-mTRPM7 S1107R plasmids using polyethyleneimine (PEI) (Polysciences, Warrington, PA) or LT1 (Mirus Bio, Madison, WI) by mixing with DNA and Dulbecco’s phosphate-buffered saline (DPBS) and adding to cells in 6-well polystyrene plates (Corning Incorporated, Kennebunk, ME) (Zhelay et al., 2018 (link)). Plasmid DNA was purified using PureYield plasmid midiprep kit (Promega, Madison, WI).

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Holderby K.G, & Kozak J.A. (2024). Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells. Frontiers in Pharmacology, 15, 1341799.

Publication 2024

PEI 6-well polystyrene plates PureYield plasmid midiprep kit

Corresponding Organization :

Other organizations : Division of Undergraduate Education

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Variable analysis

independent variables

Transfection method: polyethyleneimine (PEI) or LT1

dependent variables

Expression of pEGFP-mTRPM7 and pEGFP-mTRPM7 S1107R plasmids

control variables

Dulbecco's phosphate-buffered saline (DPBS)
6-well polystyrene plates (Corning Incorporated, Kennebunk, ME)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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