For Arabidopsis, the extraction of total RNA from roots or shoots and the RT-qPCR experiments were performed as previously described [50 (link)]. RNAseq experiments were performed as previously described [43 (link)].
For plant crops, Nucleospin 8 RNA kit (Macherey–Nagel) was used to isolate the RNA from the different plant species tested (50 mg root powder/sample). The quality and concentration of all samples were checked using 4200 Tapestation (Agilent Technologies), followed by DNase treatment and cDNA synthesis from 1 µg of RNA (iScriptTM gDNA). RT-qPCR reactions were performed in a Real-Time PCR Detection System (BIO-RAD) using a total of 10 µl reaction containing 5 µL of Universal SYBR Green Supermix (BIO-RAD) and primers at 0.5 µM. All reactions were performed in technical triplicates. Primers were designed with Primer3. The list of all primers used is provided in Table S3 . Relative expression changes were calculated by the ΔCCq method. The exponential expression is calculated as 2−ΔCq, in which ΔCq is the difference between the Cq of the phosphate starvation induced gene analyzed and the average of Cq obtained from all housekeeping genes used. Values were then normalized to corresponding control.
For plant crops, Nucleospin 8 RNA kit (Macherey–Nagel) was used to isolate the RNA from the different plant species tested (50 mg root powder/sample). The quality and concentration of all samples were checked using 4200 Tapestation (Agilent Technologies), followed by DNase treatment and cDNA synthesis from 1 µg of RNA (iScriptTM gDNA). RT-qPCR reactions were performed in a Real-Time PCR Detection System (BIO-RAD) using a total of 10 µl reaction containing 5 µL of Universal SYBR Green Supermix (BIO-RAD) and primers at 0.5 µM. All reactions were performed in technical triplicates. Primers were designed with Primer3. The list of all primers used is provided in Table S
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