Bone Volume and Alveolar Measurements

Maxillae were fixed and scanned as previously described (37 (link)). The examiners were blinded to the origin of the samples. Three-dimensional images were generated and rotated with a standard orientation. Bone volume fraction was measured as the percentage of bone volume within the total volume (ROI) using AnalyzePro software (AnalyzeDirect, Inc., Overland Park, KS). Additionally, three liner measurements of the distance from the cemento-enamel junction (CEJ) to the alveolar bone crest (ABC) were taken for the first and second molars as described previously (38 (link), 39 (link)).

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Li Y., Lu Z., Kirkwood C.L., Kirkwood K.L., Wank S.A., Li A.J., Lopes-Virella M.F, & Huang Y. (2023). GPR40 Deficiency Worsens Metabolic Syndrome-Associated Periodontitis in Mice. Journal of periodontal research, 58(3), 575-587.

Publication 2023

AnalyzePro

Corresponding Organization : Ralph H. Johnson VA Medical Center

Other organizations : University at Buffalo, State University of New York, Roswell Park Comprehensive Cancer Center, National Institute of Diabetes and Digestive and Kidney Diseases, Washington State University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Bone volume fraction (percentage of bone volume within the total volume, ROI)
Distance from the cemento-enamel junction (CEJ) to the alveolar bone crest (ABC) for the first and second molars

control variables

Examiners were blinded to the origin of the samples
Three-dimensional images were generated and rotated with a standard orientation

controls

No positive or negative controls were explicitly mentioned

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