Clonal cultures of two Cryptomonas species were established from single cells isolated manually from natural habitats by glass pipetting: C. curvata KR (FBCC300012D), from Cheongyang, Korea (36° 30′ N, 126° 47′ E), and C. paramecium KR from freshwater, Daejeon, Korea (36° 21′ 57″ N, 127° 20′ 20″ E). The strains have been deposited in, and are available from, the Freshwater Bioresources Culture Collection at the Nakdong-gang National Institute of Biological Resources and the Protist Culture Collection, Department of Biology, Chungnam National University, Korea. The two cultures were grown in AF-6 medium [42 ] with distilled water and were maintained at 20°C under a 14:10 light:dark cycle with 30 μmol photons m−2 s−1 from cool white fluorescent tubes. Cultivation of C. curvata CCAP979/52 and Cryptomonas sp. CCAC1634B was carried out as described [16 (link)].
Genomic DNAs were extracted from C. paramecium KR and C. curvata KR (FBCC300012D) using the QIAGEN DNEasy Blood Mini Kit (QIAGEN, Valencia, CA, USA) following the manufacturer’s instructions. DNA extractions for C. curvata CCAP979/52 and Cryptomonas sp. CCAC1634B were done using a standard SDS-phenol/chloroform extraction method. For C. curvata CCAP979/52, organelle DNA-enriched fractions (i.e., plastid, mitochondrion, and nucleomorph) were purified as described previously [11 (link)].
Genomic DNAs were extracted from C. paramecium KR and C. curvata KR (FBCC300012D) using the QIAGEN DNEasy Blood Mini Kit (QIAGEN, Valencia, CA, USA) following the manufacturer’s instructions. DNA extractions for C. curvata CCAP979/52 and Cryptomonas sp. CCAC1634B were done using a standard SDS-phenol/chloroform extraction method. For C. curvata CCAP979/52, organelle DNA-enriched fractions (i.e., plastid, mitochondrion, and nucleomorph) were purified as described previously [11 (link)].
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