Comprehensive Immunohistochemical Analysis of FFPE Tissue

Immunohistochemical stains were performed on formalin-fixed, paraffin-embedded (FFPE) tissue sections following routine
procedures on a Ventana Benchmark automated stainer (Roche Ventana Medical Systems, Tucson, AZ), using the antibodies against CD20
(L26, Roche Diagnostics), CD3 (2GV6, Ventana), CD79a (SP18, Roche Diagnostics), PAX-5 (SP34, Roche Diagnostics), LMP1 (CS
1–4, Dako), EBNA2 (PE2, Abcam), (CD138 (B-838, Roche Diagnostics), kappa (rabbit, Roche Diagnostics), lambda (rabbit,
Roche Diagnostics), CD38 (SPC32, Vector), MUM-1 (MRQ-43, Roche Diagnostics), Ki-67 (MIB-1, Dako), IgG (rabbit, Dako), IgM (rabbit,
Dako), and IgA (rabbit, Dako). Epstein-Barr virus-encoded small RNA (EBER) in situ hybridization was performed as previously
described ^{13 (link)}.

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Gong S., Crane G.M., McCall C.M., Xiao W., Ganapathi K.A., Cuka N., Davies-Hill T., Xi L., Raffeld M., Pittaluga S., Duffield A.S, & Jaffe E.S. (2018). Expanding the Spectrum of EBV-positive Marginal Zone Lymphomas-A Lesion Associated with Diverse Immunodeficiency Settings. The American journal of surgical pathology, 42(10), 1306-1316.

Publication 2018

Ventana Benchmark CD20(L26, CD3 (2GV6, CD79a (SP18, PAX-5 (SP34, LMP1 (CS1–4, (CD138 (B-838, MUM-1 (MRQ-43, Ki-67 (MIB-1,

Antibodies Cd138 Cd79a Diagnostics Embedded paraffin Formalin In situ hybridization Mib 1 Rabbit Stains Vector Virus rna

Corresponding Organization : Center for Cancer Research

Other organizations : Johns Hopkins University

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Variable analysis

independent variables

Antibodies against CD20
CD79a
PAX-5
EBNA2
CD138
Kappa
Lambda
MUM-1
Ki-67

dependent variables

Immunohistochemical staining patterns

control variables

Formalin-fixed, paraffin-embedded (FFPE) tissue sections
Ventana Benchmark automated stainer (Roche Ventana Medical Systems, Tucson, AZ)
Epstein-Barr virus-encoded small RNA (EBER) in situ hybridization

positive controls

Not specified

negative controls

Not specified

Annotations

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