Protein isolation from liver tissue and Western blot analysis were performed as previously described (Dou et al., 2018 (link)). The following antibodies were used: anti-phosphorylated-AMPKα (Thr172), anti-AMPKα, anti-PPARα, anti-CPT-1, anti-acetyl-CoA-carboxylase (ACC), anti-phosphorylated-ACC, anti-SREBP-1c, anti-fatty acid synthase (FAS), anti-DGAT-2, anti-P53, anti-Bcl2, anti-Bax, anti-TLR4, anti-JNK, anti-phosphorylated-JNK, anti-phosphorylated-P38, anti-P38, anti-phosphorylated-ERK, anti-ERK, anti-cleaved-caspase-3, and anti-GAPDH (Cell Signalling Technology, Danvers, MA, United States).
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