Molecular Signaling Pathway Plasmids

The expression plasmids of cGAS, STING, RIG-I N, TBK1, IRF3, IRF3-5D, IκB-sr, HA-Ub-WT and HA-Ub-K0, and luciferase reporter plasmids IFNB-Luc, IRF3-Luc, TK-Luc have been described previously [44 (link),46 (link),81 (link)]. The EBV protein expression library was kindly provided by Lori Frappier from University of Toronto, and the p2089 bacmid was provided by Wolfgang Hammerschmidt from German Center for Infection Research (Munich, Germany). The expression constructs of BPLF1 mutant C61A, HA-Ub-K63, HA-Ub-K48, HA-Ub-K27, HA-Ub-K63R, HA-Ub-K48R were constructed by Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Lenti-MPHv2 and lenti-SAMv2 plasmids [45 (link)] were gifts from Feng Zhang from Broad Institute.

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Lui W.Y., Bharti A., Wong N.H., Jangra S., Botelho M.G., Yuen K.S, & Jin D.Y. (2023). Suppression of cGAS- and RIG-I-mediated innate immune signaling by Epstein-Barr virus deubiquitinase BPLF1. PLOS Pathogens, 19(2), e1011186.

Publication 2023

Q5 Site-Directed Mutagenesis Kit

Cgas Gifts Ifnb Infection Irf3 Library Lori Luciferase Plasmids Protein Rig i Site directed mutagenesis Tbk1

Corresponding Organization : Tung Wah College

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Variable analysis

independent variables

STING
RIG-I N
IRF3-5D
IκB-sr
HA-Ub-WT
HA-Ub-K0
BPLF1 mutant C61A
HA-Ub-K63
HA-Ub-K48
HA-Ub-K27
HA-Ub-K63R
HA-Ub-K48R

dependent variables

IFNB-Luc
IRF3-Luc
TK-Luc

control variables

Lenti-MPHv2
Lenti-SAMv2

positive controls

EBV protein expression library

negative controls

P2089 bacmid

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