Metabolic Enzyme Activities in Rice

Activities of enzymes related to C metabolism, including PEPC (Osuna et al., 1996 (link)),ERS (Ratinaud et al., 1983 (link)), TPS (Goddijn et al., 1997 (link)), and SPS (Feng et al., 2019 (link)) in rice tissues were assayed (detailed information is shown in Supplementary material M1).
Activities of enzymes activated in N metabolism, namely, NR (Ahanger et al., 2021 (link)), nitrite reductase (NiR) (Lin et al., 2022a (link)), and GS (Hou et al., 2019 (link)) in rice tissues were determined (detailed information is shown in Supplementary material M1).
Activities of enzymes involved in 2-OG biosynthesis, i.e., NADP-ICDH (Gálvez et al., 1994 (link)), isocitrate dehydrogenases (NAD-IDH) (Gálvez et al., 1994 (link)), and glutamate dehydrogenases (GDH) (Turano et al., 1996 (link)), were also measured (detailed information is shown in Supplementary material M1).

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Feng Y.X., Yang L., Lin Y.J., Song Y, & Yu X.Z. (2023). Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress. Frontiers in Plant Science, 14, 1086098.

Publication 2023

Biosynthesis Enzymes activities Glutamate dehydrogenases Isocitrate dehydrogenases Metabolism Nadp Nitrite reductase Rice Tissues

Corresponding Organization : Guilin University of Technology

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Variable analysis

independent variables

Activities of enzymes related to C metabolism, including PEPC, ERS, TPS, and SPS in rice tissues
Activities of enzymes activated in N metabolism, namely, NR, nitrite reductase (NiR), and GS in rice tissues
Activities of enzymes involved in 2-OG biosynthesis, i.e., NADP-ICDH, isocitrate dehydrogenases (NAD-IDH), and glutamate dehydrogenases (GDH)

dependent variables

Enzyme activities measured in rice tissues

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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