Immunostaining of T. gondii Infection

Serological reagents used were: Anti-IIGP1 165 rabbit antiserum [35 (link)], anti-IIGP1 10E7, and 10D7 mouse monoclonal antibodies (mAb), anti-IGTP I68120 mAb (BD Transduction Laboratories, Lexington, Kentucky, United States), anti-TGTP1 A20 goat antiserum (Santa Cruz Biotechnology, Santa Cruz, California, United States), anti-LRG-47 A19 goat antiserum (Santa Cruz), anti-GTPI H53 rabbit antiserum raised against the N-terminal peptide MEEAVESPEVKEFEY, anti-IRG-47 2078 rabbit antiserum raised against the peptides CKTPYQHPKYPKVIF, and CDAKHLLRKIETVNVA, anti-T. gondii rabbit antiserum (BioGenex, San Ramon, California, United States), anti-LAMP1 1D4B rat mAb (University of Iowa, Iowa City, Iowa, United States), anti-GRA7 5–241–178 mouse mAb (gift from R. Ziemann, Abbott Laboratories, Abbot Park, Illinois, United States) [41 (link)], anti-ROP2/3/4 T24A7 mouse mAb (gift of J. Dubremetz, Montpellier, France) [59 (link)], anti-ctag1 2600 rabbit antiserum raised against the peptide CLKLGRLERPHRD, anti-ERP60 rabbit antiserum (gift from T. Wileman, BBSRC, Pirbright, United Kingdom), SPA-265 anti-calnexin rabbit antiserum (Stressgene), anti-PDI mAb (BD Transduction Laboratory), anti-Gm130 mAB (BD Transduction Laboratory), goat anti-mouse Alexa 546/488, goat anti-rabbit Alexa 546/488, donkey anti-goat Alexa 546/488, donkey anti-mouse Alexa 488, donkey anti-rabbit Alexa 488, donkey anti-rat Alexa 488, goat anti-rabbit Alexa 680 (Molecular Probes, Eugene, Oregon, United States).

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Martens S., Parvanova I., Zerrahn J., Griffiths G., Schell G., Reichmann G, & Howard J.C. (2005). Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases. PLoS Pathogens, 1(3), e24.

Publication 2005

Anti antibodies Anti t Antiserum Calnexin Donkey Erp60 Goat Lamp1 Molecular probes Mouse Peptide Rabbit

Corresponding Organization :

Other organizations : University of Cologne, Max Planck Society, Max Planck Institute for Infection Biology, European Molecular Biology Laboratory, Heinrich Heine University Düsseldorf

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Protocol cited in 8 other protocols

Variable analysis

independent variables

Anti-IIGP1 165 rabbit antiserum
Anti-IIGP1 10E7 mouse monoclonal antibody
Anti-IIGP1 10D7 mouse monoclonal antibody
Anti-IGTP I68120 mouse monoclonal antibody
Anti-TGTP1 A20 goat antiserum
Anti-LRG-47 A19 goat antiserum
Anti-GTPI H53 rabbit antiserum
Anti-IRG-47 2078 rabbit antiserum
Anti-T. gondii rabbit antiserum
Anti-LAMP1 1D4B rat monoclonal antibody
Anti-GRA7 5–241–178 mouse monoclonal antibody
Anti-ROP2/3/4 T24A7 mouse monoclonal antibody
Anti-ctag1 2600 rabbit antiserum
Anti-ERP60 rabbit antiserum
SPA-265 anti-calnexin rabbit antiserum
Anti-PDI mouse monoclonal antibody
Anti-Gm130 mouse monoclonal antibody
Goat anti-mouse Alexa 546/488
Goat anti-rabbit Alexa 546/488
Donkey anti-goat Alexa 546/488
Donkey anti-mouse Alexa 488
Donkey anti-rabbit Alexa 488
Donkey anti-rat Alexa 488
Goat anti-rabbit Alexa 680

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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